Research Scientist - Hplc Job Description

HPLC for cholesterol determination

HPLC is being used to determine cholesterol in food and tissue extracts. HPLC is not used in routine clinical analyses performed with automated clinical multiple analysis systems based on colorimetric or fluorometric assays. Sterols can be separated by GC, but not by HPLC.

One HPLC system in widespread use can determine the cholesterol's concentration a 300 3.9mm internal diameter Bondapak C18 column with a 100% methanol mobile phase and a variable wavelength ultraviolet detector set at 230 nm. The two techniques yield the same results when determining cholesterol in a variety of foods. HPLC is popular in forensic science laboratories.

Some of the same materials can be analyzed by HPLC. HPLC is the method of choice for separating analytes that are sensitive to temperature, such as explosives, which can break violently when subjected to heat. Below are some of the more popular applications to evidence analysis.

The visible and UV spectrum of ink and dyes can be used to compare writing instruments to writing on a document. It can be used to follow the aging of the ink as it ages. Fiber dyes can be separated by HPLC.

Qualitative Analysis of Scientific Research

Scientific research can be classified in many ways. The data collection techniques can be used to make a classification based on causality, time and the medium through which they are applied. The Quasi-Experimental Research is conducted in cases where a quick result is requested and the participants or research areas cannot be randomized.

A hypothesis the first step in clinical research. A hypothesis a claim about the value of a population. There are two types of hypotheses.

The planning phase begins after the determination of a hypothesis. A protocol is a clinical research plan. The reasons for research, number and qualities of participants, tests to be applied, study duration and what information to be gathered from the participants should be developed are some of the reasons for a protocol.

Clinical research is done on a sample and a population. The number of samples should be determined correctly. The sample size formulas are used based on the method to be used.

The sample size increases error probability decreases. The sample size is calculated based on the hypothesis. The state in which statistical difference has clinically significance is called ES.

The National Institute of Environmental Health Sciences

There are non-governmental organizations that deal with the value and policy implications of science and technology. Publicly advocating for specific policies related to one's research through editorials, letters to the editor, public speeches, media interviews, etc. Scientists can often diffuse or alleviate charges that their work is biased by discussing their value assumptions and commitments when drawing policy implications from their research.

They should distinguish between what their data and results show and what they infer from their data. Scientists should be aware of how their involvement in controversial issues may affect the public perception of their work. The National Institute of Environmental Health Sciences is headed by David B. Resnik.

HPLC: A New Technique for Analytical Chemistry

The question needs to be answered with a simple answer. HPLC is analytical technique used to separate components of an organic mixture of compounds when they are nonvolatile, thermally unstable, and have highmolecular weights. Separation sciences have contributed to the growth and understanding of analytical chemistry and have helped control the quality of consumer products.

Credit goes to chromatographic techniques and HPLC. You need to have a good understanding of the HPLC principles and parts to generate the highest reliability data. A conceptual understanding of the function of each component will add to your comfort level with your HPLC system and ensure long term usage with high reliance on output data.

Anyone who works in a laboratory is likely to use an HPLC at some point. You may have to rely on HPLC to answer some important questions after you finish your degree. Most of us believe that we can run the software and inject samples.

The areas of research and product development have been made more interesting by the contributions of chromatography. HPLC is used in many applications, including pharmaceuticals, foods, biochemical research, synthetic polymers, studies on bio-molecules, and environmental monitoring. The scope of HPLC is expanding at an astonishing pace and many new applications are being reported in reputed research journals almost daily.

It is possible to analyze a sample over a wide concentration range. The analysis done for sample amounts ranging from a few hundredths of a gram to a few hundredths of a gram. The ability of HPLC to handle samples with lowmolecular weights has resulted in the development of synthetic polymers and large bio-molecules.